Players can obtain it on the ::Train area, buying it for 750 train points. They are a popular secondary option for players who don't yet have the loyalty points for the loyalty gloves (Anguish Gloves, Berserker Gloves, Mystic Gloves) for range, melee, and mage respectively or the Space Boxing Gloves, which are a combination of all three loyalty gloves.
Currently, it is not dropped by any Bosses.
They can currently be obtained from the following Boxes:
At the moment, the price of American Boxing Gloves is roughly: 900K-1M DSCoins